Vitrification and Thawing Media

Kitazato vitrification media are the most versatile option for cryopreservation in your laboratory.

Increase your efficiency by using the same media for vitrification and warming of oocytes and embryos, in all their stages of development, from zygote stage to blastocyst.

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The Cryotop® Method offers the same products for both oocyte and embryo vitrification.

This helps to standardize procedures, optimize laboratory routine and improve clinical results.

VT601 / VT601US – Vitrification Media

  • 1 x 1.5 ml Basic Solution vial (BS)
  • 1 x 1.5 ml Equilibration Solution vial (ES)
  • 2 x 1.5 ml Vitrification Solution vial (VS)

VT602 / VT602US – Thawing Media

  • 2 x 4 ml Thawing Solution vial (TS)
  • 1 x 4 ml Diluent Solution vial (DS)
  • 1 x 4 ml Washing Solution vial (WS)

Kitazato media’s composition is entirely synthetic. Among their components, the following are notable: 

  • Hydroxypropyl cellulose (HPC) prevents the risk of contamination, increases the survival rate in hatched blastocysts and reduces mechanical stress during warming.
  • Trehalose functions as an osmotic agent in place of sucrose. It provides greater safety in the process, improving the protection of the cellular membranes.
  • DMSO in combination with ethylene glycol in the media assures less toxicity and the best outcomes after warming. 
  • The incorporation of gentamicin prolongs the sterility of the media, guaranteeing greater safety in handling the solutions. 

Kitazato media’s composition is entirely synthetic. Among their components, the following are notable: 

  • Hydroxypropyl cellulose (HPC) prevents the risk of contamination, increases the survival rate in hatched blastocysts and reduces mechanical stress during warming.
  • Trehalose functions as an osmotic agent in place of sucrose. It provides greater safety in the process, improving the protection of the cellular membranes.
  • DMSO in combination with ethylene glycol in the media assures less toxicity and the best outcomes after warming. 
  • The incorporation of gentamicin prolongs the sterility of the media, guaranteeing greater safety in handling the solutions. 

Kitazato media composition works effectively at room temperature, allowing convenient exchange between water and CPAs and preserving an intact cell membrane. 

The only step that needs to be performed at 37°C is the first one of warming procedure. The temperatures difference between the liquid nitrogen at -196°C and the first solution at 37°C is the warming rate, which is essential for the success of the procedure.

Product Update

Vitrification and Thawing Media Shelf Life Extended

Read more

How to perform a succesfull vitrification

Embryo Vitrification and Thawing Protocol

 

Order Number Code Product Name Quantity Regulatory
91101 VT601 Vitrification Media
1BS, 1ES, 2VS		 4 x 1.5ml MDR CE (2797)
91121 VT602

Thawing Media
2TS, 1DS, 1WS

 4 x 4ml MDR CE (2797)
91255 VT602UF -TSx4

Ultra-fast Warm
4TS

 4 x 4ml MDR CE (2797)

Order Number Code Product Name Quantity
91103 VT601US

Vitrification Media
1BS, 1ES, 2VS

 4 x 1.5ml
91130 VT602US

Thawing Media
2TS, 1DS, 1WS

 4 x 4ml
91244 VT602US-TSx4

Thawing Media
4TS

 4 x 4ml

Please note that products may only be available in selected countries.

Vitrification Product Catalog - EMEA Only

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Vitrification Product Catalog - USA Only

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Vitrification Product Catalog - ASIA Only

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Vitrification Flyer

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Vitrification Protocol

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Vitrification Media VT601 IFU - MDR UE

Download

Thawing Media VT602 IFU - MDR UE

Download

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